Coding

Part:BBa_K3482022:Design

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)


Azurin from Pseudomonas aeruginosa PAO1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 253
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized to E. coli ATCC 8739 and cleared of BsaI and BsmBI restriction sites using Genome Calligrapher (https://christenlab.ethz.ch/GenomeCalligrapher/upload_form_v6.php)


Source

The azurin gene comes from Pseudomonas aeoruginosa PAO1

References